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plenti destination vectors  (Addgene inc)


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    Structured Review

    Addgene inc plenti destination vectors
    Plenti Destination Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenti+pgk+neo+dest/pLenti+PGK+Neo+DEST+(w531-1)+(Plasmid+%2319067)/bio_rxiv__2025__06__11__659056-187-11-14
    Average 93 stars, based on 48 article reviews
    plenti destination vectors - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Long-term breast cancer response to CDK4/6 inhibition defined by TP53-mediated geroconversion.
    Article Snippet: .. For another guide RNA, we obtained sgTP53_3which was a gift fromWilliamHahn (Addgene plasmid # 78164), and lentiCRISPRv2 hygro which was a gift from Brett Stringer (Addgene plasmid # 98291). pDONR223_MDM2_WT was a gift from Jesse Boehm & Matthew Meyerson & David Root (Addgene plasmid # 82897) cloned into pLenti PGK Neo DEST (pLenti PGK Neo DEST (w531-1) a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 19067) by using the Gateway LR Clonase II Enzyme Mix (Invitrogen). pDONR223_CDKN1A_WT was a gift from Jesse Boehm &William Hahn & David Root (Addgene plasmid # 82201) was cloned into pInducer20 (a gift from Stephen Elledge, Addgene plasmid # 44012) by using the Gateway LR Clonase II Enzyme Mix. ..

    Article Title: Long-term breast cancer response to CDK4/6 inhibition defined by TP53-mediated geroconversion
    Article Snippet: .. For another guide RNA, we obtained sgTP53_3 which was a gift from William Hahn (Addgene plasmid # 78164), and lentiCRISPRv2 hygro which was a gift from Brett Stringer (Addgene plasmid # 98291). pDONR223_MDM2_WT was a gift from Jesse Boehm & Matthew Meyerson & David Root (Addgene plasmid # 82897) cloned into pLenti PGK Neo DEST (pLenti PGK Neo DEST (w531–1) a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 19067) by using the Gateway LR Clonase II Enzyme Mix (Invitrogen). pDONR223_CDKN1A_WT was a gift from Jesse Boehm & William Hahn & David Root (Addgene plasmid # 82201) was cloned into pInducer20 (a gift from Stephen Elledge, Addgene plasmid # 44012) by using the Gateway LR Clonase II Enzyme Mix. ..

    Article Title: BLM and BRCA1-BARD1 coordinate complementary mechanisms of joint DNA molecule resolution.
    Article Snippet: .. For lentiviral transductions, Spot-BARD1 cDNAs were inserted into pLenti PGK Neo DEST (a gift from Eric Campeau and Paul Kaufman134; Addgene plasmid # 19067) using Gateway cloning. .. GFP-SLX4 was amplified by PCR from pcDNA5/FRT/TO-GFP-SLX4 (a gift from John Rouse21) and inserted into a modified TLCV2 vector (a gift from Philip Hublitz) to replace the Cas9 cassette using In-Fusion cloning (Takara Bio) according to the manufacturer’s instructions.

    Clone Assay:

    Article Title: Long-term breast cancer response to CDK4/6 inhibition defined by TP53-mediated geroconversion.
    Article Snippet: .. For another guide RNA, we obtained sgTP53_3which was a gift fromWilliamHahn (Addgene plasmid # 78164), and lentiCRISPRv2 hygro which was a gift from Brett Stringer (Addgene plasmid # 98291). pDONR223_MDM2_WT was a gift from Jesse Boehm & Matthew Meyerson & David Root (Addgene plasmid # 82897) cloned into pLenti PGK Neo DEST (pLenti PGK Neo DEST (w531-1) a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 19067) by using the Gateway LR Clonase II Enzyme Mix (Invitrogen). pDONR223_CDKN1A_WT was a gift from Jesse Boehm &William Hahn & David Root (Addgene plasmid # 82201) was cloned into pInducer20 (a gift from Stephen Elledge, Addgene plasmid # 44012) by using the Gateway LR Clonase II Enzyme Mix. ..

    Article Title: Long-term breast cancer response to CDK4/6 inhibition defined by TP53-mediated geroconversion
    Article Snippet: .. For another guide RNA, we obtained sgTP53_3 which was a gift from William Hahn (Addgene plasmid # 78164), and lentiCRISPRv2 hygro which was a gift from Brett Stringer (Addgene plasmid # 98291). pDONR223_MDM2_WT was a gift from Jesse Boehm & Matthew Meyerson & David Root (Addgene plasmid # 82897) cloned into pLenti PGK Neo DEST (pLenti PGK Neo DEST (w531–1) a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 19067) by using the Gateway LR Clonase II Enzyme Mix (Invitrogen). pDONR223_CDKN1A_WT was a gift from Jesse Boehm & William Hahn & David Root (Addgene plasmid # 82201) was cloned into pInducer20 (a gift from Stephen Elledge, Addgene plasmid # 44012) by using the Gateway LR Clonase II Enzyme Mix. ..

    Mutagenesis:

    Article Title: Invasion of glioma cells through confined space requires membrane tension regulation and mechano-electrical coupling via Plexin-B2.
    Article Snippet: The mutations I436C & S993C (“Lock1”) and I436C & T1051C (“Lock2”) were introduced into a CRISPR-resistant PLXNB2 cDNA plasmid by site directed mutagenesis (Thermo). .. The mutant cDNAs were transferred by Gateway reaction into pLENTI PGK Neo DEST (Addgene #19067; deposited by Eric Campeau & Paul Kaufman). .. The lock mutant plasmids have been deposited at Addgene (lock1: #182879 and lock2: #182880).

    Article Title: Invasion of glioma cells through confined space requires membrane tension regulation and mechano-electrical coupling via Plexin-B2
    Article Snippet: The mutations I436C & S993C (“Lock1”) and I436C & T1051C (“Lock2”) were introduced into a CRISPR-resistant PLXNB2 cDNA plasmid by site directed mutagenesis (Thermo). .. The mutant cDNAs were transferred by Gateway reaction into pLENTI PGK Neo DEST (Addgene #19067; deposited by Eric Campeau & Paul Kaufman). .. The lock mutant plasmids have been deposited at Addgene (lock1: #182879 and lock2: #182880).

    Cloning:

    Article Title: BLM and BRCA1-BARD1 coordinate complementary mechanisms of joint DNA molecule resolution.
    Article Snippet: .. For lentiviral transductions, Spot-BARD1 cDNAs were inserted into pLenti PGK Neo DEST (a gift from Eric Campeau and Paul Kaufman134; Addgene plasmid # 19067) using Gateway cloning. .. GFP-SLX4 was amplified by PCR from pcDNA5/FRT/TO-GFP-SLX4 (a gift from John Rouse21) and inserted into a modified TLCV2 vector (a gift from Philip Hublitz) to replace the Cas9 cassette using In-Fusion cloning (Takara Bio) according to the manufacturer’s instructions.

    other:

    Article Title: Long-term breast cancer response to CDK4/6 inhibition defined by TP53-mediated geroconversion
    Article Snippet: pLenti PGK Neo DEST , Campeau et al., 2009 , Addgene# 19067.



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